Biosynthesis of gold and selenium nanoparticles by purified protein from *Acinetobacter* sp. SW30

Wadhwani, Sweety A.; Shedbalkar, Utkarsha U.; Singh, Richa; Chopade, Balu A.

doi:10.1016/j.enzmictec.2017.10.007

Biosynthesis of gold and selenium nanoparticles by purified protein from Acinetobacter sp. SW30

Authors

Sweety A. Wadhwani

Utkarsha U. Shedbalkar

Richa Singh

Balu A. Chopade

Published

April 1, 2018

Abstract

Synthesis of nanoparticles is an enzymatic reduction process in microorganisms. In the present study, a protein, lignin peroxidase has been purified by DEAE-Cellulose anion exchange chromatography and Biogel P-150 gel filtration chromatography from the cell suspension of Acinetobacter sp. SW30 responsible for the synthesis of gold nanoparticles (AuNP) and selenium nanoparticles (SeNP). The purified fraction has a specific activity of 29.4 U/mg/min with 959 fold purification. Native and SDS PAGE confirmed that purified lignin peroxidase is monomeric enzyme with 97.4 KDa molecular weight. The enzyme synthesized spherical crystalline AuNP (10 ± 2 nm) and amorphous SeNP (100 ± 10 nm). It has maximum activity at pH 2 and temperature 40 °C, with 1.0 mM Km value, when n-propanol was used as a substrate. Activity was completely inhibited by sodium thiosulphate and zinc sulphate. This is the first report on association of lignin peroxidase in the synthesis of AuNP and SeNP from Acinetobacter sp. SW30.

Wadhwani SA, Shedbalkar UU, Singh R, Chopade BA (2018). Enzyme and Microbial Technology 111: 81–86.

https://doi.org/10.1016/j.enzmictec.2017.10.007

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Citation

BibTeX citation:

@article{wadhwani2018,
  author = {Wadhwani, Sweety A. and Shedbalkar, Utkarsha U. and Singh,
    Richa and Chopade, Balu A.},
  title = {Biosynthesis of Gold and Selenium Nanoparticles by Purified
    Protein from {*Acinetobacter*} Sp. {SW30}},
  journal = {Enzyme and Microbial Technology},
  volume = {111},
  pages = {81-86},
  date = {2018-04},
  url = {https://drrichasingh.in/papers/2018-aunp-senp-purified-protein.html},
  doi = {10.1016/j.enzmictec.2017.10.007},
  issn = {0141-0229},
  langid = {en},
}

For attribution, please cite this work as:

Wadhwani, Sweety A.; Shedbalkar, Utkarsha U.; Singh, Richa; Chopade, Balu A. 2018. Biosynthesis of gold and selenium nanoparticles by purified protein from Acinetobacter sp. SW30. Enzyme and Microbial Technology 111 (April): 81–86. https://doi.org/10.1016/j.enzmictec.2017.10.007.