Antibacterial Activities of Bacteriagenic Silver Nanoparticles Against Nosocomial Acinetobacter baumannii

Authors

Richa Singh

Jeet Vora

Shradhda B Nadhe

Sweety A Wadhwani

Utkarsha U Shedbalkar

Balu A Chopade

Published

June 1, 2018

Abstract
Acinetobacter baumannii has emerged as one of the major nosocomial pathogens implicated in variety of severe infections and mortality. It is rapidly developing multi-drug resistance and also possesses surface colonization ability, which make it most difficult to treat through traditional antibiotics. This is an extensive study to describe the antibacterial activity of bacteriagenic silver nanoparticles (AgNPs) against A. baumannii AIIMS 7 in planktonic and biofilm mode. Minimum inhibitory concentration of antibiotics were in the range of 1 to 4096 μg/ml whereas AgNPs inhibited planktonic bacteria at concentration of 16 μg/ml. Fractional inhibitory concentration index revealed the synergistic interaction of AgNPs with doxycycline, tetracycline and erythromycin. Nanoparticles exhibited significant biofilm disruption activity with minimum biofilm eradication concentration of 2 mg/ml. Eradication of mature biofilm was enhanced on exposure to combination of AgNPs and antibiotics. These nanoparticles affected bacterial growth and distorted cellular morphology. Intracellular oxidative stress, induced in presence of AgNPs, also rendered bacteria susceptible to killing by nanoparticles. Besides this, AgNPs were found to interact with thiol-groups, which indicate their potential to interact with cellular proteins to exhibit antimicrobial activity.

Singh R, Vora J, Nadhe SB, Wadhwani SA, Shedbalkar UU, Chopade BA (2018). Journal of Nanoscience and Nanotechnology 18(6): 3806–3815.

https://doi.org/10.1166/jnn.2018.15013

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Citation

BibTeX citation:
@article{singh2018,
  author = {Singh, Richa and Vora, Jeet and Nadhe, Shradhda B and
    Wadhwani, Sweety A and Shedbalkar, Utkarsha U and Chopade, Balu A},
  title = {Antibacterial {Activities} of {Bacteriagenic} {Silver}
    {Nanoparticles} {Against} {Nosocomial} {*Acinetobacter} Baumannii*},
  journal = {Journal of Nanoscience and Nanotechnology},
  volume = {18},
  number = {6},
  pages = {3806-3815},
  date = {2018-06-01},
  url = {https://drrichasingh.in/papers/2018-agnp-nosocomial-baumannii.html},
  doi = {10.1166/jnn.2018.15013},
  issn = {1533-4880},
  langid = {en},
}
For attribution, please cite this work as:
Singh, Richa; Vora, Jeet; Nadhe, Shradhda B; Wadhwani, Sweety A; Shedbalkar, Utkarsha U; Chopade, Balu A. 2018. Antibacterial Activities of Bacteriagenic Silver Nanoparticles Against Nosocomial Acinetobacter baumannii. Journal of Nanoscience and Nanotechnology 18 (6): 3806–15. https://doi.org/10.1166/jnn.2018.15013.