Chemical and biological metal nanoparticles as antimycobacterial agents: A comparative study

Authors

Richa Singh

Laxman U. Nawale

Manisha Arkile

Utkarsha U. Shedbalkar

Sweety A. Wadhwani

Dhiman Sarkar

Balu A. Chopade

Published

August 1, 2015

Abstract
Resistance among mycobacteria leading to multidrug-resistant and extensively drug-resistant tuberculosis is a major threat. However, nanotechnology has provided new insights in drug delivery and medicine development. This is the first comparative report to determine the activity of chemically and biologically synthesised silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mycobacteria. Screening data revealed the high mycobactericidal efficiency of AgNPs, with minimum inhibitory concentrations (MICs) of <3 μg/mL, whereas no such activity was exhibited by AuNPs at concentrations up to 100 μg/mL. Moreover, in vitro and ex vivo THP-1 infection model assays showed greater efficacy of chemical AgNPs compared with biogenic AgNPs to inhibit active and dormant stage mycobacterial growth. Up to 40% cytotoxicity against human cell lines was observed at a AgNP concentration of 10× MIC (30 μg/mL) after 48 h. AgNPs were shown to have more specificity towards mycobacteria than towards other Gram-negative and Gram-positive pathogenic bacteria. The selectivity index was found to be in the range of 11–23, indicating the potential of these nanoparticles for use in developing new therapeutics for tuberculosis.

International Journal of Antimicrobial Agents 46(2): 183-188 (2015).

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https://doi.org/10.1016/j.ijantimicag.2015.03.014

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Citation

BibTeX citation:
@article{singh2015,
  author = {Singh, Richa and Nawale, Laxman U. and Arkile, Manisha and
    Shedbalkar, Utkarsha U. and Wadhwani, Sweety A. and Sarkar, Dhiman
    and Chopade, Balu A.},
  title = {Chemical and Biological Metal Nanoparticles as
    Antimycobacterial Agents: {A} Comparative Study},
  journal = {International Journal of Antimicrobial Agents},
  volume = {46},
  number = {2},
  pages = {183-188},
  date = {2015-08},
  url = {https://drrichasingh.in/papers/2015-metal-np-antimycobacterial.html},
  doi = {10.1016/j.ijantimicag.2015.03.014},
  issn = {0924-8579},
  langid = {en},
}
For attribution, please cite this work as:
Singh, Richa; Nawale, Laxman U.; Arkile, Manisha; Shedbalkar, Utkarsha U.; Wadhwani, Sweety A.; Sarkar, Dhiman; Chopade, Balu A. 2015. Chemical and biological metal nanoparticles as antimycobacterial agents: A comparative study. International Journal of Antimicrobial Agents 46 (2): 183–88. https://doi.org/10.1016/j.ijantimicag.2015.03.014.