Novel polyhedral gold nanoparticles: green synthesis, optimization and characterization by environmental isolate of Acinetobacter sp. SW30

Authors

Sweety A. Wadhwani

Utkarsha U. Shedbalkar

Richa Singh

Meena S. Karve

Balu A. Chopade

Published

July 1, 2014

Abstract
Gold nanoparticles have enormous applications in cancer treatment, drug delivery and nanobiosensor due to their biocompatibility. Biological route of synthesis of metal nanoparticles are cost effective and eco-friendly. Acinetobacter sp. SW 30 isolated from activated sewage sludge produced cell bound as well as intracellular gold nanoparticles when challenged with HAuCl4 salt solution. We first time report the optimization of various physiological parameters such as age of culture, cell density and physicochemical parameters viz HAuCl4 concentration, temperature and pH which influence the synthesis of gold nanoparticles. Gold nanoparticles thus produced were characterized by various analytical techniques viz. UV–Visible spectroscopy, X-ray diffraction, cyclic voltammetry, transmission electron microscopy, selected area electron diffraction, high resolution transmission electron microscopy, environmental scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy and dynamic light scattering. Polyhedral gold nanoparticles of size 20 ± 10 nm were synthesized by 24 h grown culture of cell density 2.4 × 109 cfu/ml at 50 °C and pH 9 in 0.5 mM HAuCl4. It was found that most of the gold nanoparticles were released into solution from bacterial cell surface of Acinetobacter sp. at pH 9 and 50 °C.

Wadhwani SA, Shedbalkar UU, Singh R, Karve MS, Chopade BA (2014). World Journal of Microbiology and Biotechnology 30(10): 2723–2731.

https://doi.org/10.1007/s11274-014-1696-y

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Citation

BibTeX citation:
@article{wadhwani2014,
  author = {Wadhwani, Sweety A. and Shedbalkar, Utkarsha U. and Singh,
    Richa and Karve, Meena S. and Chopade, Balu A.},
  title = {Novel Polyhedral Gold Nanoparticles: Green Synthesis,
    Optimization and Characterization by Environmental Isolate of
    {*Acinetobacter*} Sp. {SW30}},
  journal = {World Journal of Microbiology and Biotechnology},
  volume = {30},
  number = {10},
  pages = {2723-2731},
  date = {2014-07-01},
  url = {https://drrichasingh.in/papers/2014-polyhedral-aunp-sw30.html},
  doi = {10.1007/s11274-014-1696-y},
  issn = {1573-0972},
  langid = {en},
}
For attribution, please cite this work as:
Wadhwani, Sweety A.; Shedbalkar, Utkarsha U.; Singh, Richa; Karve, Meena S.; Chopade, Balu A. 2014. Novel polyhedral gold nanoparticles: green synthesis, optimization and characterization by environmental isolate of Acinetobacter sp. SW30. World Journal of Microbiology and Biotechnology 30 (10): 2723–31. https://doi.org/10.1007/s11274-014-1696-y.